Chromosome conformation capture of Megalops atlanticus - SRA

SRX14005086: Chromosome conformation capture of Megalops atlanticus
1 ILLUMINA (Illumina NovaSeq 6000) run: 109.1M spots, 32.7G bases, 10.2Gb downloads

Design: Hi-C data was generated using the Arima-HiC kit (Ref. 510008), according to the manufacturer's protocols using 10l of blood as starting material, the Truseq DNA PCR-Free kit and Truseq DNA UD Indexes (Illumina, ref. 20015962, ref. 20020590) and the KAPA library Amplification kit (Roche, ref. KK2620). Hi-C library was sequenced in paired end 2*150nt mode on Novaseq6000 (Illumina), using half a lane of a SP flow cell (ref. 20027464). Image analyses and base calling were performed using the Illumina NovaSeq Control Software and Real-Time Analysis component (v3.4.4). Demultiplexing was performed using Illumina's conversion software (bcl2fastq v2.20). The quality of the raw data was assessed using FastQC (v0.11.8) from the Babraham Institute and the Illumina software SAV (Sequencing Analysis Viewer). Potential contaminants were investigated with the FastQ Screen (v0.14.0) software from the Babraham Institute (Wingett & Andrews, 2018)

Submitted by: INRAE (Fish Physiology and Genomics laboratory)

Study: Megalops atlanticus isolate:YG-15Mar2019-1 Genome sequencing and assembly

PRJNA690086 • SRP357698 • All experiments • All runs

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Genome sequencing of Megalops Atlanticus

Sample: Blood for HiC

SAMN25277672 • SRS11843259 • All experiments • All runs

Organism: Megalops atlanticus

Library:

Name: Megalops_atlanticus_HiC

Instrument: Illumina NovaSeq 6000

Strategy: Hi-C